Preliminary phytochemical screening of six medicinal plants used in traditional medicine manjulika yadav, sanjukta chatterji, sharad kumar gupta and geeta watal alternative therapeutics unit, drug development division, medicinal research lab, department of chemistry, university of allahabad, allahabad211 002, u. Studies on the antimicrobial properties and phytochemical. The methanolic and aqueous extracts of dried wild edible mushroom were analyzed for phytochemical screening, antibacterial, antioxidant activity dpph and total phenolic content. Qualitative phytochemical analysis of garlic extracts indicated the presence of flavonoids, alkaloids, tannins, phenols, saponins, terpenoids, steroids and phytosterols in ethanol and hot water. Antifungal properties and phytochemical screening of extracts of african basil ocimum gratissimum l. For volatile oil estimation 50 mg of powdered material crude drug is taken and subjected to hydro distillation. Initial phytochemical screening of the different parts of mansoa alliacea l. Pdf the present study investigates the qualitative and quantitative analysis of the major bioactive constituents of medicinally important plant. Phytochemical screening of developing garlic and effect of its aqueous extracts on viability of cardiac cell line. Pdf there seems to be little literature about the toxicity of the widely used garlic, which makes it important to investigate chemical elements as.
Review of literature phytochemical screening medicinal plants are best owed with large number of pharmaceutically useful compounds which can be studied for investigation of new drugs for many serious diseases like cancer, tumours, aids and many human degenerative diseases. Phytochemical and physicochemical characterization of allium sativum l. Phytochemical screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial parts of a. Garlic has been used as a functional food to inhibit the growth of pathogens but also served as a remedy for the prevention of a number of diseases. Phytochemicals screening and antioxidant effectiveness of garlic allium sativum from timor island the people of timor island only know garlic as a kitchen spice. Results of the phytochemical analysis of garlic and onion varieties.
Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq 2 department of plant, soil and agricultural systems, plant biotechnology and. Fresh garlic samples were separated into cloves and skin and then freezedried at. A phytochemical screening of the extracts was also carried out to determine the constituents of garlic. The phytochemical screening of the garlic for various phytochemical constituents was conducted using laboratory method. Dragendorf test for alkaloids, sodium hydroxide test for flavonoids, ferric chloride test for tannins. Phytochemicals screening and antioxidant effectiveness of. Study of phytochemical analysis and antioxidant activity of allium sativum of bundelkhand region vandana singh1, ramesh kumar2 1m. In vitro bioactivity and phytochemical screening of. The phytochemical screening of the garlic for various phytochemical constituents such as terpenoids, flavonoids, alkaloids, reducing sugars, steroid, glycoside, phenol, anthraquinones, saponin and tannin was conducted using standard methods as described by sofowora 12 and trease and evans. Phytochemical screening and antioxidant activity of clove. Phytochemical and antioxidant analyses of selected edible mushrooms, ginger and garlic from ebonyi state, nigeria. Preliminary phytochemical screening of 27 plants species.
A comparative study pavni k, esha b, neha j, tushar a, shrey k, suchit a, sarita a, vibha r and,neeraj w department of biotechnology, jaypee institute of information technology university, a10 sec 62, noida, uttar pradesh, india. In our study garlic scales were screened for various phytochemicals namely coumarines, quinines, phlobotannnins, tannins, phenols, cardiac glycosides, anthraquinones, glycosides, steroids, terpenoids, etc. The qualitative phytochemical screening of allium sativum aqueous and ethanol. Department of plant biology, university of ilorin, pmb 1515, ilorin, nigeria issued april 1, 2010 abstract this study evaluates the antimicrobial properties of garlic allium sativum extracts on three fungi. Phytochemical screening of medicinal plants research. Student, department of biochemistry, bundelkhand university, jhansi, india 2associate prof.
Phytochemical screening by ftir spectroscopic analysis of leaf extracts of selected indian medicinal plants r. Phytochemical screening, elemental analysis and acute. Phytochemical screening of garlic extract phytochemical screening was done in order to detect the presence of bioactive constituents such as alkaloids, tannins, saponins, phenols, glycosides, phlobatannins, flavonoids and glycosides using the methods described by sofowora 1978, trease and evans 1989. Phytochemical screening of the leaf extract of moringa oleifera revealed the presence of alkaloids, glycosides, phenols, saponins, tannins, volatile oils and hydrolysable tannins. The phytochemical constituents and proximate composition of onion allium cepa l. They observed that, the plant is known to possess antiseptic, analgesic, antiinflammatory, antimicrobial, antistress, immunomodulatory, hypoglycemic, hypertensive and antioxidant properties. Preliminary phytochemical screening, quantitative estimation of total flavonoids, total phenols and antioxidant activity of ephedra alata decne. Nidal jaradat 1, fatima hussen1 and anas al ali 2 1department of pharmacy, faculty of medicine and health sciences, annajah national university nablus, state of palestine. Phytochemical screening and antimicrobial studies on the. Phytochemical screening was carried out according to the method of trease and evans, total flavonoid content was measured by the aluminium chloride colorimetric assay and total phenolic content was estimated spectrophotometrically by folinciocalteau method. The objective of the study was to investigate the phytochemical constituents and proximate composition of garlic a. Venkatachalam pg and research department of microbiology, sengunthar arts and science college, thiruchengode637205, namakkal, india corresponding author a b s t r a c t article info accepted. Table 2 and 3 showed the diameter of zone of inhibition of the extracts at different concentrations. Preliminary phytochemical screening of different solvent extracts of selected lndian spices v.
Phytochemical analysis and antimicrobial effect of lemon grass cymbopogon citratus obtained from zaria, kaduna state, nigeria. The content of phenolic compounds free and bound forms in the garlic skin samples was significantly p garlic cloves, and was also higher p garlic. The phytochemical composition of allium cepa allium. The results of phytochemical screening showed the presence of some chemical groups in. Preliminary phytochemical screening revealed the presence of tannin, flavonoid, carbohydrate, protein, saponin, alkaloid and glycoside in the plants while anthraquinone was absent. Phytochemical analysis of medicinal plants occurring in.
Phytochemical characteristics and antimicrobial activity of. Phytochemical screening by ftir spectroscopic analysis of. Measurement of antioxidant activity using dpph method. Phytochemical screening and antimicrobial studies on the methanolic bulb extract of allium sativum l. Preliminary phytochemical screening of crude extracts from. Evaluation of the phytochemical properties and antifungal. Preliminary phytochemical screening, quantitative analysis.
Phytochemical analysis of medicinal plants occurring in local area of mardan abdul wadood 1, mehreen ghufran, syed babar jamal, muhammad naeem, ajmal khan 2, rukhsana ghaffar3. Foods free fulltext phytochemical characteristics and. Preliminary phytochemical screening, quantitative estimation. Medicinal plants are the local heritage with global importance. Phytochemical importance of medicinal plants as potential sources of anticancer agents himani raina1, garima soni1, nupur jauhari1, neelam sharma2, navneeta bharadvaja1, 1department of biotechnology, delhi technological university, delhi, india 2tissue culture and cryopreservation unit, national bureau of plant genetic resources, new delhi, india. Phytochemical screening of petroleum ether, chloroform, ethanol, aqueous and hydroalcoholic extracts revealed the presence flavonoids, tannins triterpenoids, saponins, sterols, alkaloids and carbohydrates by positive reaction with the respective test reagent. Phytochemical evaluation and pharmacological screening of. Phytochemical screening of developing garlic and effect of. From the qualitative findings presented in table 1, it is observed that the citrus paradisi of different extracts confirmed the presence of alkaloids, flavonoids, reducing sugars, flavanoids, phenols, proteins, amino acids.
Indoles, isothiocyanates, and sulforaphane from vegetables, such as broccoli, alylic. The phytochemical tests were conducted using standard methods of analysis. The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of anabasis aphylla l. In these screening process alkaloids, tannins, saponins, flavonoids and terpenoids, glycosides, phenols. The cold maceration and agar well diffusion technique were employed to assess phytochemical properties and the antimicrobial potency of cymbopogon citratus lemongrass leaf extracts against selected bacterial pathogens using different solvents. Study of phytochemical analysis and antioxidant activity of. Results of the phytochemical characterization of the seven varieties tested are presented in table 3. Experiment 1 suitability of extraction solvent through phytochemical screening of garlic initial phytochemical screening of the methanolic, ethanolic and aqueous extract of garlic powder showed ethanol to be the most suitable solvent for the extraction of active chemical ingredients from the fresh garlic bulb. Preliminary phytochemical screening of different solvent.
Phytochemical screening and promiximate composition and onion. Pdf phytochemical and antioxidant analyses of selected. Photochemical tests were carried out by standard methods 6 2. Qualitative phytochemical analysis of garlic allium sativum. Now thats a name that doesnt exactly roll off the tongue. Use the link below to share a fulltext version of this article with your friends and colleagues. Phytochemical screening and antibacterial and antifungal. In vitro bioactivity and phytochemical screening of selected spices used in mauritian foods. View phytochemical screening of medicinal plants research papers on academia.
Phytochemical screening for identification of bioactive. Preliminary phytochemical screening of 27 plants species use in ethnoveterinary in khartoum state, sudan. For cancer chemoprevention, curcumin turmeric, capsaicin chili peppers, lycopene tomatoes, resveratrol grapes, sulforaphane broccoli, and allicin garlic are noted as anticancer phytochemicals from fruits and vegetables. The content of phenolic compounds free and bound forms in the garlic skin samples was significantly p of the garlic cloves, and was also higher p garlic. All the methods were performed by standard protocols. Phytochemical screening of the different extracts showed that garlic contains important compounds such as carbohydrates, reducing sugars, lipids, flavonoids. Allium cepa, allium sativum, phytochemical screening, essential oils. Numerous extraction methods were carried out such as soxhlet, cold extractions and hot extractions.
Aug 23, 2019 commercial nonaustralian grown garlic product of china was purchased from a local supermarket in brisbane, queensland, australia, and was included for comparison. Phytochemical screening and proximate analysis of garlic. The light scaly leaves on the onion bulbs were removed and the fleshy parts chopped into tiny pieces, dried in an aircirculating oven in the laboratory and ground into fine powder using a mechanical grinder. Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq. Garlic improves the elasticity of blood vessels and increasing blood circulation.
The method used to test secondary metabolites was phytochemical screening using color reagents. Both the aqueous and methanol extract of garlic were observed. When raw garlic is crushed a strong oxidant allicin is formed which has strong antibiotic property. Phytochemical characteristics and antimicrobial activity. The phytochemical screening showed that it contained alkaloids. Antifungal properties and phytochemical screening of.
The result of the phytochemical screening showed the presence of saponin. Discussion indomethacin is an established ulcerogenic agent especially in an empty stomach john et al, 1990 mostly on the glandular mucosal part of stomach. Phytochemical is a term that refers to a variety of plantderived compounds with therapeutic activities such as anticarcinogenic, antimutagenic, antiinflammatory, and antioxidant properties mcguire, 2011. The preliminary phytochemical results showed the occurrence of active compounds such as carbohydrate, glycosides, phytosterol s, phenol, tannin s, f lavanoids, alkaloid, terpenoids and saponins. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Phytochemical screening and antioxidant activity of. Preliminary phytochemical analysis of the extracts of. Phytochemical and physicochemical characterization of allium. Phytochemicals screening revealed the results that alkaloids, reducing sugar. Phytochemical and ethnobotanical evaluation of garlic bulb allium. Why phytochemicals are important uc davis integrative medicine. Phytochemical screening the phytochemical screening of the garlic bulb extract was carried out according to the procedures described by trease and evans 1989.
Abstract in the present study, preliminary phytochemical screening of 27plants used in ethnoveterinary medicine was. Initial phytochemical screening of the different parts of. Pdf phytochemical screening, elemental analysis and acute. Phytochemical and physicochemical characterization of. General techniques involved in phytochemical analysis. Pdf phytochemical analysis and antimicrobial effect of. Ramaswamy department of zoology, karpagam university, coimbatore, tamilnadu, india corresponding author abstract introduction a large number of medicinal plants are used as alternate medicine for diseases of.
Preliminary phytochemical screening, quantitative analysis of. Garlic were purchased at sokoto central market in may, 2002 and identified by traditional herbalist. Studies on the antimicrobial properties and phytochemical screening of garlic allium sativum extracts olusanmi, m. Phytochemical is a broad term meaning plant phyto chemical referring to a wide variety of compounds that occur naturally in plants.
However, detailed followup studies are warranted with more samples, quantity, and cultivars, in order to elucidate the potential of phytochemical rich garlic for domestic and industrial applications e. Phytochemical analysis of aqueous extract of ocimum sanctum. But if you are interested in healthy eating, you should know some of the following phytochemical basics. Qualitative phytochemical analysis of garlic allium. Phytochemical screening of aqueous extract of garlicalliumsativum bulbs article pdf available january 2014 with 12,647 reads how we measure reads. Proximate analysis, minerals, and heavy metals of edible garlic cloves. Pdf phytochemical screening of aqueous extract of garlic. The preliminary phytochemical investigation was carried out with ethanolic extract of cloves of allium sativum for quantitative identification of photochemical constituents. Testing the effectiveness of free radical inhibitors from garlic ethanol extract from timor island was carried out in 2 stages. Study of phytochemical analysis and antioxidant activity of allium. Garlic helps to reduces blood pressure and reduce blood lipid levels. Phytochemical screening and proximate analysis of garlic allium.
General techniques involved in phytochemical analysis international journal of advanced research in chemical science ijarcs page 29 11. Phytochemical evaluation and pharmacological screening of antiparkinsons activity of allium 2 this research work was conducted to evaluate the efficacy of an ethanolic extract of allium sativum in the treatment of haloperidol induced catalepsy, for antiparkinson activity. This study evaluates the antimicrobial properties of garlic allium sativum extracts on three fungi namely aspergillus flavus, curvularia lunata and fusarium moniliforme using the pour plate method. Materials and methods plant material the bulbs of a. General techniques involved in phytochemical analysis international journal of advanced research in chemical science ijarcs page 27 mass is packed and the top of the percolator is closed. Department of neurochemistry, national institute of mental health and neuro sciences 1, bangalore, karnataka, india. If you continue browsing the site, you agree to the use of cookies on this website.
The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. Tech, centre for biotechnology, department of chemical engineering, an dhra university, visakhapatnam03, andhra pradesh, india 1. This research provides new knowledge of the benefits of garlic in the health sector, especially as an inhibitor of free radical that can trigger various degenerative diseases. Phytochemical importance of medicinal plants as potential. Preliminary phytochemical screening revealed the presence of tannin, flavonoid, carbohydrate, protein, saponin, alkaloid and glycoside in the plants while. Preliminary phytochemical screening of plants is also necessary for the discovery and development of novel therapeutic agents with improved efficacy10,11.
Phytochemical screening this is a technique that allows the presence of groups of chemical families in active extracts of a plant substance to be demonstrated in order to evaluate the antibacterial activity. Phytochemical screening the phytochemical screening of the extract of the plant by organic solvent was carried out using standard qualitative procedures. Phytochemical screening the phytochemical screening of the garlic for various phytochemical constituents such as terpenoids, flavonoids, alkaloids, reducing sugars, steroid, glycoside, phenol, anthraquinones, saponin and tannin was conducted using standard methods as described by sofowora 12 and trease and evans. Tannins, flavonoids, trepenoids, alkaloids and saponin, carbohydrates, protein, and cardiac glycosides were found in the extracts while anthraquinones was absent. The phytochemical screening of garlic extract revealed the presence of saponin, terpenoid, flavonoids, volatile oil, amino acid and protein and cardiac glycosides. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay.
1534 1607 1136 1528 1323 711 1563 1009 884 836 859 1569 722 813 748 399 255 1640 214 1356 164 702 1201 563 837 501 1573 1168 219 1 286 387 414 323 1143 481 1492 440 773 544